NUCLEAR-MAGNETIC-RESONANCE STUDIES OF HYALURONAN (HA) - EVIDENCE OF COMPETITIVE-INHIBITION OF INTERCHAIN ASSOCIATIONS BY PHOSPHOLIPIDS WHICH MAY RESULT IN DECREASED ANTIINFLAMMATORY AND CARTILAGE PROTECTING PROPERTIES OF HA

Authors
GHOSH P HUTADILOK N LENTINI A
Citation
P. Ghosh et al., NUCLEAR-MAGNETIC-RESONANCE STUDIES OF HYALURONAN (HA) - EVIDENCE OF COMPETITIVE-INHIBITION OF INTERCHAIN ASSOCIATIONS BY PHOSPHOLIPIDS WHICH MAY RESULT IN DECREASED ANTIINFLAMMATORY AND CARTILAGE PROTECTING PROPERTIES OF HA, Heterocycles, 38(8), 1994, pp. 1757-1774
Citations number
40
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Heterocycles → ACNP
ISSN journal
03855414
Volume
38
Issue
8
Year of publication
1994
Pages
1757 - 1774
Database
ISI
SICI code
0385-5414(1994)38:8<1757:NSOH(->2.0.ZU;2-U
Abstract
The chain flexibility of solutions of hyaluronan (HA) of different mol ecular weights was determined by H-1-nmr spectroscopy in the absence a nd presence of the phospholipid Dipalmitoyl DL-alpha-phosphatidylcholi ne (DPC). Sonication of high or low molecular weight HA with DPC for p eriods up to 60 min markedly increased HA chain flexibility as determi ned by observing the spin-spin relaxation times for the glucosamine ac etyl group protons. From these data it is proposed that DPC competes f or those hydrophobic centres along the HA chain which are normally res ponsible for the inter and intra chain interactions and which confer s tiffness to the HA molecule.