PREFERENTIAL USE OF DILUTIONS OF SINGLE SERA THAN MIXTURE OF SERA TO STANDARDIZE THE QUANTITATION OF ANTICARDIOLIPIN ANTIBODIES

The aim of this study was to compare four house standards coming from two University Hospital laboratories with the standards provided by th e Antiphospholipid Standardization Laboratory (ASL) in order to quanti fy anticardiolipin antibodies. Using two different plates and two diff erent buffered protein solutions, slopes from the serial dilutions of each of the four house standards were found comparable. In contrast di fferent slopes were obtained when using the ASL standards which consis t of a mixture of sera. Our results indicate that dilutions of single sera are more suitable than mixture of sera when quantification of ant icardiolipin antibodies is required.