SOLVENT STUDY ON THE 9-SUBSTITUTED QUINOLIZINOCOUMARINS USED AS PRECOLUMN FLUORESCENT AND CHEMILUMINESCENT REAGENTS IN LIQUID-CHROMATOGRAPHY

Citation
F. Traore et al., SOLVENT STUDY ON THE 9-SUBSTITUTED QUINOLIZINOCOUMARINS USED AS PRECOLUMN FLUORESCENT AND CHEMILUMINESCENT REAGENTS IN LIQUID-CHROMATOGRAPHY, Analytica chimica acta, 294(1), 1994, pp. 75-84
Citations number
25
Categorie Soggetti
Chemistry Analytical
Journal title
ISSN journal
00032670
Volume
294
Issue
1
Year of publication
1994
Pages
75 - 84
Database
ISI
SICI code
0003-2670(1994)294:1<75:SSOT9Q>2.0.ZU;2-B
Abstract
9-substitued fluorescent quinolizinocoumarin derivatives (luminarins) were extensively studied spectroscopically, because of the wide variat ion in the emissions observed during liquid chromatographic detection in different mobile phases. Photophysical parameters measured at room temperature (lambda(ex), lambda(em), lambda(max), nu(a)(')-nu(f)('), S tokes' shift, relative quantum yield of fluorescence (phi(f)), singlet level energy, molar absorptivity, etc.) were measured for eleven quin olizinocoumarin derivatives in twelve solvents. The observed spectrosc opic variations associated with the change in solvents were analysed i n terms of modification in the microenvironment of the luminarin deriv atives. For this, a multiple linear regression analysis was performed in the linear solvation energy relationships model. The same regressio n model was used to describe and demonstrate the correlation between t he structure of 9-sided chains and the observed spectral modifications . Finally, the practical consequences of such a study allowed the infl uence of the microenvironment on the fluorescence detection of luminar in derivatives after normal and reversed phase liquid chromatography t o be demonstrated.