F. Traore et al., SOLVENT STUDY ON THE 9-SUBSTITUTED QUINOLIZINOCOUMARINS USED AS PRECOLUMN FLUORESCENT AND CHEMILUMINESCENT REAGENTS IN LIQUID-CHROMATOGRAPHY, Analytica chimica acta, 294(1), 1994, pp. 75-84
9-substitued fluorescent quinolizinocoumarin derivatives (luminarins)
were extensively studied spectroscopically, because of the wide variat
ion in the emissions observed during liquid chromatographic detection
in different mobile phases. Photophysical parameters measured at room
temperature (lambda(ex), lambda(em), lambda(max), nu(a)(')-nu(f)('), S
tokes' shift, relative quantum yield of fluorescence (phi(f)), singlet
level energy, molar absorptivity, etc.) were measured for eleven quin
olizinocoumarin derivatives in twelve solvents. The observed spectrosc
opic variations associated with the change in solvents were analysed i
n terms of modification in the microenvironment of the luminarin deriv
atives. For this, a multiple linear regression analysis was performed
in the linear solvation energy relationships model. The same regressio
n model was used to describe and demonstrate the correlation between t
he structure of 9-sided chains and the observed spectral modifications
. Finally, the practical consequences of such a study allowed the infl
uence of the microenvironment on the fluorescence detection of luminar
in derivatives after normal and reversed phase liquid chromatography t
o be demonstrated.