Hp. Baum et al., EXPRESSION OF PROLIFERATION-ASSOCIATED PROTEINS (PROLIFERATING CELL NUCLEAR ANTIGEN AND KI-67 ANTIGEN) IN BOWENS-DISEASE, British journal of dermatology, 131(2), 1994, pp. 231-236
Bowen's disease and invasive squamous cell carcinoma arising in Bowen'
s disease (Bowen's carcinoma) were investigated immunohistochemically
with regard to their expression of proliferating cell nuclear antigen
(PCNA) and the Ki-67 antigen. Both proliferation-associated proteins w
ere found in all layers of the tumours, and were present in nuclei of
any size and shape. The nucleoli lacked PCNA immunoreactivity, but the
y expressed the Ki-67 antigen. The PCNA labelling index was 36.9 +/- 1
2.6% in Bowen's disease (n = 16), and 40.7 +/- 11.0% in Bowen's carcin
oma (n = 3). The Ki-67 antigen labelling index was 43.6 +/- 16.7% in B
owen's disease (n=16), and 51.7 +/- 11.6% in Bowen's carcinoma (n = 3)
. Dyskeratotic cells were positive for PCNA and the Ki-67 antigen, sug
gesting that these cells are not in a post-mitotic state. We conclude
that in dyskeratotic cells of Bowen's disease the cell cycle is interr
upted by nuclear disorganization.