VITALITY OF EIMERIA-TENELLA AFTER IRRADIA TION INSULT

An irradiation insult as attenuation of Eimeria tenella oocysts with g amma Co-80 irradiation in a dosis of 250 Gy does not influence the tem poral lapse and the rate of the sporulation. The excystation exhibit n on changes with the rate of average 77,0 per cent opposite the untreat ed controls with 76,1 per cent. In reproducible trials fresh excystate d sporozoits of radioattenuated oocysts demonstrate a 100 per cent abi lity of life by the vital colouring with methylene blue. Sporozoits of radioattenuated and fullvirulent oocysts have the same pattern of dis tribution of amylopectin as a sign of vitality. With increasing durati on of storage the ability of survival decreases quantitative in like m anner as well as in irradiated and nonirratiated sporozoits in the cor relation with the reserve of energy for the duration of 6 days.