PCR ANALYSIS TO DISTINGUISH BETWEEN ALLELES OF A MEMBER OF A MULTIGENE FAMILY CORRELATED WITH WHEAT BREAD-MAKING QUALITY

Authors
DOVIDIO R ANDERSON OD
Citation
R. Dovidio et Od. Anderson, PCR ANALYSIS TO DISTINGUISH BETWEEN ALLELES OF A MEMBER OF A MULTIGENE FAMILY CORRELATED WITH WHEAT BREAD-MAKING QUALITY, Theoretical and Applied Genetics, 88(6-7), 1994, pp. 759-763
Citations number
20
Categorie Soggetti
Genetics & Heredity
Journal title
Theoretical and Applied Genetics → ACNP
ISSN journal
00405752
Volume
88
Issue
6-7
Year of publication
1994
Pages
759 - 763
Database
ISI
SICI code
0040-5752(1994)88:6-7<759:PATDBA>2.0.ZU;2-W
Abstract
Good or poor wheat bread-making quality is associated with two allelic pairs at the Glu-DI complex locus, designated 1Dx5-1Dy10 and 1Dx2-1Dy 12, respectively. The polymerase chain reaction (PCR) verified the pre sence of the HMW-glutenin 1Dx5 gene from genomic DNA extracted from pa rt of the endosperm of a single dry seed, or a small amount of leaf or root tissue, of several bread-wheat cultivars. This easy, quick, and non-destructive PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting bread-wheat gen otypes with good bread-making properties.