CHARACTERIZATION OF PLASMIDS FROM DIKETOGLUCONIC ACID-PRODUCING STRAINS OF GLUCONOBACTER-OXYDANS

Authors
VERMA V FELDER M CULLUM J QAZI GN
Citation
V. Verma et al., CHARACTERIZATION OF PLASMIDS FROM DIKETOGLUCONIC ACID-PRODUCING STRAINS OF GLUCONOBACTER-OXYDANS, Journal of biotechnology, 36(1), 1994, pp. 85-88
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Journal of biotechnology → ACNP
ISSN journal
01681656
Volume
36
Issue
1
Year of publication
1994
Pages
85 - 88
Database
ISI
SICI code
0168-1656(1994)36:1<85:COPFDA>2.0.ZU;2-Y
Abstract
Gluconobacter oxydans ATCC 9937, which produces 2,5-diketogluconic aci d, an intermediate in vitamin C synthesis, has three plasmids of sizes 27.7 kb (pVJ1), 12.3 kb (pVJ2) and 18 kb (pVJ4). A restriction map wa s constructed of pVJ1. A potential glucose dehydrogenase gene was loca ted on pVJ1 using the polymerase chain reaction with heterologous prim ers. Two other G. oxydans strains had no detectable plasmid DNA (IFO 1 2258) and a plasmid (pVJ3) of 9.4 kb (IFO 3293), respectively.