TAXOL AND CISPLATIN INHIBIT PROLIFERATION OF T47D HUMAN BREAST-CANCERCELLS

We have examined the influence of taxol and cisplatin on the rate and extent of proliferation of T47D cells grown in the presence of 5% feta l bovine serum (FBS). An 8-day exposure of the cells to taxol inhibite d the growth completely maintaining the number of cells to the level s een at initial plating. The taxol effect was evident at 10 nM - 1 mu M concentration and the half maximum inhibition was calculated to be 20 nM taxol. While the cells in the control group continued to prolifera te during the 8-day growth period, cells in the taxol-treated group re mained at the initial number. Similar observations were made regarding the influence of cisplatin, which caused 80-90% inhibition in the num ber of the cells. When combined, taxol and cisplatin caused a further inhibition in the proliferation of T47D cells. The results of our stud ies demonstrate that both taxol and cisplatin, the known antineoplasti c agents, block the proliferation of T47D human breast cancer cells ef fectively and are potentially useful chemotherapeutic agents for the m anagement of breast cancer. (C) 1994 Academic Press, Inc.