K. Isobe et al., GENE-TRANSFER OF TNF RECEPTOR FOR TREATMENT OF CANCER BY TNF, Biochemical and biophysical research communications, 202(3), 1994, pp. 1538-1542
Tumor necrosis factor-alpha receptors (TNFR-55 and TNFR-75) were inser
ted into retrovirus derived vector. They were transfected into a packa
ging cell line. The high titer of transfectants, which produced virus
containing TNFR-55 or TNFR-75, was obtained. TNFR-55 and TNFR-75 negat
ive human colon cancer cells were infected with this virus as a model
experiment of gene therapy. Although the original colon cancer cell li
ne did not express TNFR-55 or TNFR-75, the colon cancer cells, which w
ere infected by recombinant virus, expressed a high level of TNFR-55 o
r TNFR75. TNFR-55 or TNFR-75 transformed colon cancer cells were kille
d by recombinant TNF. (C) 1994 Academic Press, Inc.