GENE-TRANSFER OF TNF RECEPTOR FOR TREATMENT OF CANCER BY TNF

Tumor necrosis factor-alpha receptors (TNFR-55 and TNFR-75) were inser ted into retrovirus derived vector. They were transfected into a packa ging cell line. The high titer of transfectants, which produced virus containing TNFR-55 or TNFR-75, was obtained. TNFR-55 and TNFR-75 negat ive human colon cancer cells were infected with this virus as a model experiment of gene therapy. Although the original colon cancer cell li ne did not express TNFR-55 or TNFR-75, the colon cancer cells, which w ere infected by recombinant virus, expressed a high level of TNFR-55 o r TNFR75. TNFR-55 or TNFR-75 transformed colon cancer cells were kille d by recombinant TNF. (C) 1994 Academic Press, Inc.