SOME PHYSICOCHEMICAL AND ENZYMATIC-PROPERTIES OF SELENIUM-CONTAINING ABZYME

We successfully prepared the Se-containing abzyme (Se-abzyme) with glu tathione peroxidase (GPX) activity and futher studied its physicochemi cal and enzymic properties and stabilities. Data showed that the isoel ectric point of the abzyme was 6.95-7.08, and its molecular weight was 158 KD. The ranges of optimum pH and temperature of the Se-abzyme wer e wider than the native GPX. The store stability of the abzyme was hig her than the native GPX. The Se content in the abzyme was found to be 5 mol Se/mol abzyme by X-ray photoelectron spectrum, and binding const ant 1.11X10(7)M(-1) by using ELISA method. The Se-abzyme was inhibited competitively by dithiobis (2-nitrobenzoic acid) (DTNB), and inhibiti on constant was determined to be 1.25X10(-3)M(-1). (C) 1994 Academic P ress, Inc.