POLYPEPTIDE CONFORMATIONAL SPACE - DYNAMICS BY SOLUTION NMR DISORDER BY X-RAY CRYSTALLOGRAPHY

A symmetric dimer of a polypeptide in an organic solvent is shown to b e a useful molecular system for observing the effects of crystal packi ng and solution dynamics on structure determination by X-ray crystallo graphy and solution NMR methods. Organic solvents are not ideal models of a lipid environment, but they represent a better model environment than does water, in fact, for modeling some properties of the lipid e nvironment, organic solvents work very well. In particular, it is a go od model for assessing the influence of lipids on local polypeptide dy namics and conformational rearrangements. The solution NMR structure o f gramicidin in the mixed solvent of benzene and ethanol is compared t o the crystal structure formed from a benzene/ethanol azeotrope. High resolution structural characterization leads to unique correlations of disorder in the crystal to dynamics in solution. Furthermore, crystal packing effects lead to significant asymmetries in both the polypepti de backbone and in the side-chains.