DETECTION OF HUMAN PARVOVIRUS B19 DNA PCR PRODUCTS BY RNA PROBE HYBRIDIZATION ENZYME-IMMUNOASSAY

Authors
ERDMAN DD DURIGON EL HOLLOWAY BP
Citation
Dd. Erdman et al., DETECTION OF HUMAN PARVOVIRUS B19 DNA PCR PRODUCTS BY RNA PROBE HYBRIDIZATION ENZYME-IMMUNOASSAY, Journal of clinical microbiology, 32(9), 1994, pp. 2295-2298
Citations number
22
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
32
Issue
9
Year of publication
1994
Pages
2295 - 2298
Database
ISI
SICI code
0095-1137(1994)32:9<2295:DOHPBD>2.0.ZU;2-R
Abstract
We have developed an RNA probe hybridization enzyme immunoassay for de tection of human parvovirus B19 PCR-amplified DNA. The assay is easy t o perform and increases assay sensitivity without the added inconvenie nce and risk of false-positive results associated with nested PCR.