Lk. Crawfordmiksza et Dp. Schnurr, QUANTITATIVE COLORIMETRIC MICRONEUTRALIZATION ASSAY FOR CHARACTERIZATION OF ADENOVIRUSES, Journal of clinical microbiology, 32(9), 1994, pp. 2331-2334
A microneutralization assay that automates the reading and interpretat
ion of viral infectivity and neutralization data was developed for the
characterization of adenoviruses (AV). Virus, serum, and cells were a
dded to 96-well plates, incubated for 7 days, and stained with vital s
tain. The A(550) of solubilized dye was read in a plate reader interfa
ced to a personal computer which analyzed the results. Correlation of
A(550) values with visual observation of cytopathic effect was extreme
ly good (r = \ 0.977625 \ ). Clinical isolates of 17 AV from 11 patien
ts were characterized by colorimetric neutralization assay. Prototype
AV titers tested were comparable to those determined by tube methods.
Prototype homotypic antiserum titers were comparable to or greater tha
n those determined by standard tube neutralization.