Bd. Boyan et al., CELL BIOLOGY OF CALCIFIED TISSUES - EXPERIMENTAL-MODELS OF DIFFERENTIATION AND MECHANISMS BY WHICH LOCAL AND SYSTEMIC FACTORS EXERT THEIR EFFECTS, Connective tissue research, 34-5(1-4), 1996, pp. 117-124
Interpretation of the cell biology Literature, as it relates to format
ion and mineralization of calcifying tissues, is complicated by the pl
ethora of models available, Some culture models use heterogeneous popu
lations of cells while others use relatively homogeneous populations,
The issues are further confused by comparison of monolayer and three d
imensional cultures, In addition, transformed and nontransformed cell
Lines are also used. As little clinical data about the age and sex of
the original donor for many of these cell lines is lacking, it is impo
ssible to know where in the cell lineage the cells were when they were
isolated, yet this information can have a direct impact on the data o
btained and their interpretation, Furthermore, many responses are attr
ibuted to the cell, while much of the effect observed may be targeted
to the matrix, These issues are discussed and a potential mechanism ex
plaining how cells can modulate events in the matrix nongenomically is
presented.