EFFECT OF GLYCEROLIZATION PROCEDURE AND REMOVAL OF SEMINAL PLASMA ON POSTTHAW SURVIVAL AND GOT-RELEASE FROM BOER GOAT SPERMATOZOA

Forty ejaculates (20 for each of 2 experiments) were collected from 4 Boer goat bucks at weekly intervals to study the effect of glyceroliza tion procedure and removal of seminal plasma on progressive motility, percent live spermatozoa and release of glutamic oxaloacetic transamin ase (GOT) before and after the freezing of semen. Stepwise glyceroliza tion at 37 degrees C gave higher progressive motility and percentage o f live spermatozoa both before freezing and after thawing than onestep glyceroliza-tion at 37 degrees C or stepwise extension with glycerol being added after cooling to 5 degrees C. The GOT-release was reduced before freezing and after thawing of semen with stepwise glycerolizati on (P < 0.05). Progressive motility and the percentage of live spermat ozoa were higher (P < 0.05) after the freezing of whole semen than in washed spermatozoa. The concentration of GOT in the extra-cellular flu id was lower in washed spermatozoa prior to freezing (P < 0,05); but a fter thawing, the washed spermatozoa released more GOT than spermatozo a in whole semen. Removal of seminal plasma prior to freezing spermato zoa in an extender containing egg yolk had an unfavorable effect on th eir post-thaw motility and integrity.