C. Rey et al., CHARACTERIZATION OF THE APATITE CRYSTALS OF BONE AND THEIR MATURATIONIN OSTEOBLAST CELL-CULTURE - COMPARISON WITH NATIVE BONE CRYSTALS, Connective tissue research, 34-5(1-4), 1996, pp. 397-403
Calcium phosphate crystals deposited in the organic matrix synthesized
by chick bone osteoblasts in culture were studied by x-ray and electr
on diffraction, Fourier transform infrared spectroscopy and chemical c
omposition, The amounts of mineral phase deposited with time and the e
xtent of calcification (% of mineral phase in the tissue) were also de
termined as a function of time, as were the nature of the changes in t
he short range order of the crystals, The amount of mineral deposited
and the extent of calcification increased with time; the tissue not on
ly contained more crystals of apatite, but the extent of calcification
also increased with time as it does in vivo, After 30 days of culture
the extent of calcification in the cell culture matrix was similar to
that in late chick embryonic and early postnatal chick tibiae, The na
ture of the CO3 and HPD4 environments were similar to those found in v
ivo although the concentrations of these ions and the changes in their
concentrations with time appeared to develop more slowly in cell cult
ure than they do in vivo. However, the general overall pathway of matu
ration was similar in cell culture to that observed in vivo.