MOLECULAR BEACONS - TRIAL OF A FLUORESCENCE-BASED SOLUTION HYBRIDIZATION TECHNIQUE FOR ECOLOGICAL-STUDIES WITH RUMINAL BACTERIA

Authors
SCHOFIELD P PELL AN KRAUSE DO
Citation
P. Schofield et al., MOLECULAR BEACONS - TRIAL OF A FLUORESCENCE-BASED SOLUTION HYBRIDIZATION TECHNIQUE FOR ECOLOGICAL-STUDIES WITH RUMINAL BACTERIA, Applied and environmental microbiology, 63(3), 1997, pp. 1143-1147
Citations number
15
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
3
Year of publication
1997
Pages
1143 - 1147
Database
ISI
SICI code
0099-2240(1997)63:3<1143:MB-TOA>2.0.ZU;2-R
Abstract
Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of thes e probes to study rumen microbial ecology. Two cellulolytic species, R uminococcus albus and Fibrobacter succinogenes, were tested, Membrane and solution hybridizations gave similar results in competition experi ments with cocultures of R. albus 8 and F. succinogenes S85.