P. Schofield et al., MOLECULAR BEACONS - TRIAL OF A FLUORESCENCE-BASED SOLUTION HYBRIDIZATION TECHNIQUE FOR ECOLOGICAL-STUDIES WITH RUMINAL BACTERIA, Applied and environmental microbiology, 63(3), 1997, pp. 1143-1147
Molecular beacons are fluorescent probes developed for solution rather
than membrane hybridization. We have investigated the utility of thes
e probes to study rumen microbial ecology. Two cellulolytic species, R
uminococcus albus and Fibrobacter succinogenes, were tested, Membrane
and solution hybridizations gave similar results in competition experi
ments with cocultures of R. albus 8 and F. succinogenes S85.