Kc. Doerner et al., ASSESSMENT OF FECAL BACTERIA WITH BILE-ACID 7-ALPHA-DEHYDROXYLATING ACTIVITY FOR THE PRESENCE OF BAI-LIKE GENES, Applied and environmental microbiology, 63(3), 1997, pp. 1185-1188
Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai)
genes which encode enzymes in the bile acid 7 alpha-dehydroxylation (
7 alpha DeOH) pathway. Twelve 7 alpha DeOH-positive intestinal bacteri
al strains were assayed for 7 alpha DeOH activity, and 13 strains were
tested for hybridization with bai genes. Cholic acid 7 alpha DeOH act
ivity varied greatly (>100-fold) among these strains. Southern blot ex
periments showed that DNA prepared from 7 of 13 strains hybridized wit
h at least one of the bai genes from Eubacterium sp. strain VPI 12708.