ASSESSMENT OF FECAL BACTERIA WITH BILE-ACID 7-ALPHA-DEHYDROXYLATING ACTIVITY FOR THE PRESENCE OF BAI-LIKE GENES

Authors
DOERNER KC TAKAMINE F LAVOIE CP MALLONEE DH HYLEMON PB
Citation
Kc. Doerner et al., ASSESSMENT OF FECAL BACTERIA WITH BILE-ACID 7-ALPHA-DEHYDROXYLATING ACTIVITY FOR THE PRESENCE OF BAI-LIKE GENES, Applied and environmental microbiology, 63(3), 1997, pp. 1185-1188
Citations number
26
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
3
Year of publication
1997
Pages
1185 - 1188
Database
ISI
SICI code
0099-2240(1997)63:3<1185:AOFBWB>2.0.ZU;2-D
Abstract
Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai) genes which encode enzymes in the bile acid 7 alpha-dehydroxylation ( 7 alpha DeOH) pathway. Twelve 7 alpha DeOH-positive intestinal bacteri al strains were assayed for 7 alpha DeOH activity, and 13 strains were tested for hybridization with bai genes. Cholic acid 7 alpha DeOH act ivity varied greatly (>100-fold) among these strains. Southern blot ex periments showed that DNA prepared from 7 of 13 strains hybridized wit h at least one of the bai genes from Eubacterium sp. strain VPI 12708.