USE OF 4-NITROPHENOXYACETIC ACID FOR DETECTION AND QUANTIFICATION OF 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) ALPHA-KETOGLUTARATE DIOXYGENASEACTIVITY IN 2,4-D-DEGRADING MICROORGANISMS/

Authors
SASSANELLA TM FUKUMORI F BAGDASARIAN M HAUSINGER RP
Citation
Tm. Sassanella et al., USE OF 4-NITROPHENOXYACETIC ACID FOR DETECTION AND QUANTIFICATION OF 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) ALPHA-KETOGLUTARATE DIOXYGENASEACTIVITY IN 2,4-D-DEGRADING MICROORGANISMS/, Applied and environmental microbiology, 63(3), 1997, pp. 1189-1191
Citations number
14
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
63
Issue
3
Year of publication
1997
Pages
1189 - 1191
Database
ISI
SICI code
0099-2240(1997)63:3<1189:UO4AFD>2.0.ZU;2-E
Abstract
Purified 2,4-dichlorophenoxyacetic acid (2,4-D)/alpha-ketoglutarate di oxygenase (TfdA) was shown to use 4-nitrophenoxyacetic acid (K-m = 0.8 9 +/- 0.04 mM, k(cat) [catalytic constant] = 540 +/- 10 min(-1)), prod ucing intensely yellow 4-nitrophenol. This reagent was used to develop a rapid, continuous, colorimetric assay for the detection of TfdA and analogous activities in 2,4-D-degrading bacterial cells and extracts.