THE NEGATIVE-ION MASS-SPECTRA OF [M-H](-) IONS DERIVED FROM CAERIDIN AND DYNASTIN PEPTIDES - INTERNAL BACKBONE CLEAVAGES DIRECTED THROUGH ASP AND ASN RESIDUES
St. Steinborner et Jh. Bowie, THE NEGATIVE-ION MASS-SPECTRA OF [M-H](-) IONS DERIVED FROM CAERIDIN AND DYNASTIN PEPTIDES - INTERNAL BACKBONE CLEAVAGES DIRECTED THROUGH ASP AND ASN RESIDUES, Rapid communications in mass spectrometry, 11(3), 1997, pp. 253-258
The collision-induced spectra of [M-H](-) ions of peptides containing
Asp and Asn residues exhibit characteristic backbone cleavage ions pro
duced via the enolate anions of the Asp or Asn side chains. Such react
ions do not occur from the analogous Glu or Gin residues. Asp and Asn
residues may be distinguished because the backbone cleavage ion formed
from Asp undergoes pronounced loss of water: the corresponding loss o
f ammonia from the Asn backbone cleavage ion is less pronounced. The p
resence of Asp or Asn in a peptide usually results in the normal alpha
and beta backbone cleavage ions being minor in comparison to backbone
cleavage ions formed via the enolate anions of Asp or Asn. (C) 1997 b
y John Wiley & Sons, Ltd.