THE NEGATIVE-ION MASS-SPECTRA OF [M-H](-) IONS DERIVED FROM CAERIDIN AND DYNASTIN PEPTIDES - INTERNAL BACKBONE CLEAVAGES DIRECTED THROUGH ASP AND ASN RESIDUES

Citation
St. Steinborner et Jh. Bowie, THE NEGATIVE-ION MASS-SPECTRA OF [M-H](-) IONS DERIVED FROM CAERIDIN AND DYNASTIN PEPTIDES - INTERNAL BACKBONE CLEAVAGES DIRECTED THROUGH ASP AND ASN RESIDUES, Rapid communications in mass spectrometry, 11(3), 1997, pp. 253-258
Citations number
16
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
ISSN journal
09514198
Volume
11
Issue
3
Year of publication
1997
Pages
253 - 258
Database
ISI
SICI code
0951-4198(1997)11:3<253:TNMO[I>2.0.ZU;2-L
Abstract
The collision-induced spectra of [M-H](-) ions of peptides containing Asp and Asn residues exhibit characteristic backbone cleavage ions pro duced via the enolate anions of the Asp or Asn side chains. Such react ions do not occur from the analogous Glu or Gin residues. Asp and Asn residues may be distinguished because the backbone cleavage ion formed from Asp undergoes pronounced loss of water: the corresponding loss o f ammonia from the Asn backbone cleavage ion is less pronounced. The p resence of Asp or Asn in a peptide usually results in the normal alpha and beta backbone cleavage ions being minor in comparison to backbone cleavage ions formed via the enolate anions of Asp or Asn. (C) 1997 b y John Wiley & Sons, Ltd.