Samples of Blastocystis sp. obtained from humans, monkeys, pigs and ch
ickens were examined by scanning electron microscopy and transmission
electron microscopy to compare surface structures. The surface coat of
Blastocystis sp. cells from each host species showed some morphologic
al variations, but these were not sufficiently different to allow judg
ement to be made on speciation. The surface structure morphology appea
red similar for samples of Blastocystis sp. from the same host species
. The surface coat of the cultured human isolate of B. hominis was muc
h thinner than that of cells from fresh human faecal material, and the
cell surface appeared to be smoother and without the small projection
s seen in the fresh forms. Bacteria were frequently found in associati
on with the surface coat of Blastocystis sp. from all fresh faecal mat
erial. Possible functions of the surface coat, especially in relation
to protection against osmotic shock, are discussed.