ISOLATION AND CHARACTERIZATION OF PREANTRAL FOLLICLES FROM FETAL BOVINE OVARIES

Authors
HULSHOF SCJ FIGUEIREDO JR BECKERS JF BEVERS MM VANDENHURK R
Citation
Scj. Hulshof et al., ISOLATION AND CHARACTERIZATION OF PREANTRAL FOLLICLES FROM FETAL BOVINE OVARIES, Veterinary quarterly, 16(2), 1994, pp. 78-80
Citations number
16
Categorie Soggetti
Veterinary Sciences
Journal title
Veterinary quarterly → ACNP
ISSN journal
01652176
Volume
16
Issue
2
Year of publication
1994
Pages
78 - 80
Database
ISI
SICI code
0165-2176(1994)16:2<78:IACOPF>2.0.ZU;2-6
Abstract
A simple, mechanical method is described for the isolation of preantra l follicles from bovine foetuses of 220-280 days of gestation. On aver age, 2918 + 621 (s.d.) preantral follicles were isolated per ovary. Th e isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the numbe r of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically an d that they can be classified by their diameter.