DIFFRACTION QUALITY CRYSTALS OF PROTEIN-X FROM AZOTOBACTER-VINELANDII

Protein X from Azotobacter vinelandii has recently been shown to be ei ther a NADPH oxidase or a NADP(+) reductase that interacts specificall y with ferredoxin I. Single crystals have been obtained by vapor diffu sion from polyethylene glycol 4000 solutions containing 100 mM citrate buffer (pH 5.5). The crystals belong to space group P2(1)2(1)2 with u nit cell constants a = 68.9 Angstrom, b = 76.9 Angstrom c = 52.8 Angst rom and one molecule (M(r) 29,000) per asymmetric unit. The crystals d iffract to 2.5 Angstrom resolution.