CLONING AND CHARACTERIZATION OF A SULFITE-RESISTANCE GENE OF SACCHAROMYCES-CEREVISIAE

In this paper we describe the cloning and sequencing of the gene (SUL1 ) responsible for sulphite resistance in a Saccharomyces cerevisiae mu tant (Casalone et al., 1992). The deduced amino acid sequence predicte d that the gene codes for a zinc-finger protein with five fingers. Com parison of wild-type and mutant gene sequences demonstrated that the m utation event was a transversion from C to G; as a consequence of the mutation a histidine substituted an aspartic acid, affecting directly the fourth finger structure. The SUL1 gene sequence corresponds to tha t of FZF1 gene (Breitwieser et al., 1993) to which no function was att ributed. The sequence has been entered in the EMBL data library under Accession Number 77592.