THE NUCLEOTIDE-SEQUENCE OF TTP1, A GENE ENCODING A PREDICTED TYPE-II MEMBRANE-PROTEIN

The DNA sequence of a 2967 bp fragment located near the centromere of chromosome II, between the CEN2 and FUR4 genes, was determined. The se gment contains a new open reading frame of 1794 bp. The product encode d by the gene, designated TTP1, is a predicted type II membrane protei n of 597 amino acid residues with a short cytoplasmic NH2-terminus, a membrane-spanning region and a large COOH-terminal region containing t hree potential N-glycosylation sites. Gene disruption indicated that T TP1 is not essential for cell growth. The sequence has been deposited in the GenBank data library under Accession Number U05211.