EFFECT OF COLLAGENASE ON NERVE-FIBER TEASING

Teased fibre preparations are important in the investigation of periph eral nerve disorders, but can be a rather tedious procedure. A techniq ue is described in which pretreatment of the nerve sample with collage nase before osmication substantially simplifies the actual teasing pro cedure. The technique also does not alter the morphology or interfere with any further investigations required on teased fibres-for example, fluorescent antibody studies.