There is disagreement on the role of bacteria in the genesis and maint
enance of chronic secretory otitis media (CSOM). Extensive studies fro
m other countries report up to 40% of middle ear cultures with bacteri
al growth. For the present study, material was collected from 94 ears
of children with both clinical and tympanometric diagnoses of CSOM. Th
e samples were sent for bacteriological analysis, in there it was stai
ned according to Gram's method and put into growing media: MacConkey (
for gram-negative bacteria) and blood agar (for gram-positive bacteria
) for 24 h, at 37 degrees C. If germs were identified by Gram's method
, antibiograms would be carried out as well, with the Mueller Hington
medium. No cultures were made for anaerobes. Only one ear had grown ba
cteria (Staphylococcus epidermidis), which was deemed contamination. N
o other ear studied bore bacteria. The possible causes for this disagr
eement between our study and the literature are discussed.