THE ANALYSIS OF COCAINE AND ITS METABOLITES BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY (LC APCI-MS)/

The method for simultaneous determination of cocaine and its four meta bolites (benzoylecgonine, ecgonine methyl ester, ecgonine and norcocai ne) in urine by liquid chromatography/atmospheric pressure chemical io nization-mass spectrometry (LC/APCI-MS) was studied. The mass spectra showed the quasi-molecular ions, [M + H](+) as the base peak. LC/APCI- MS analysis was performed by focusing the characteristic ions at m/= 1 86, 290, 200, 304 and 290 for ecgonine, benzoylecgonine, ecgonine meth yl ester, cocaine and norcocaine, respectively. Cocaine and its four m etabolites were well separated by high performance liquid chromatograp hy (HPLC). The recoveries of cocaine and its metabolites from the spik ed urine were 40.3-94.7% by solid-phase extraction with two type cartr idges (Bond Elut Certify and Bond Elut SCX).