USE OF AN OPTICAL-FIBER-BASED BIOSENSOR TO STUDY THE INTERACTION OF BLOOD PROTEINS WITH SOLID-SURFACES

An optical fiber-based laser biosensor has been used to investigate ad sorption of blood lipoproteins and immunoglobulin G to the glass optic al-fiber surface. While lipoproteins readily interact (adsorb) with un modified optical-fiber surface, immunoglobulin G does not bind to the glass surface at a concentration of 5 mug/mL. However, upon glass surf ace modification with silane, immunoglobulin G binds significantly to the optical fiber. High-density lipoproteins and low-density lipoprote ins show a differential interaction with the glass surface, and their interaction was altered upon modification of the glass surface with ch olesterol. With these results in mind, the utility of the biosensor fo r the study of protein interaction with biorelevant surfaces has been outlined.