USE OF YARROWIA-LIPOLYTICA HEXOKINASE FOR THE QUANTITATIVE-DETERMINATION OF TREHALOSE 6-PHOSPHATE

This paper describes a procedure for the quantitative determination of trehalose 6-phosphate (T6P) based on its ability to inhibit hexokinas e from Yarrowia lipolytica. The assay is linear between 1 nmol and at least 8 nmol. The concentration of T6P in wild-type Saccharomyces cere visiae (0.15 mM) and in ras2 mutants (0.25 mM) remained unchanged in t he exponential or stationary phase of growth or after heat shock. A tp s1 mutant affected in T6P synthase did not show detectable T6P. Heat s hock increased the concentration of T6P in Schizosaccharomyces pombe f rom 0.43 to 0.75 mM.