IN-VITRO EFFECT OF 5-FLUOROURACIL, VERAPAMIL AND HYPERTHERMIA ON THE HUMAN PANCREATIC ADENOCARCINOMA CELL-LINE ASPC-1

The purpose of this study was to assess the efficacy of verapamil (20 mu M) and hyperthermia (42 degrees C) as modifiers of 5-fluorouracil ( 5-FU) used at different concentrations, in inhibiting the growth of hu man pancreatic adenocarcinoma cells. Combined verapamil and hypertherm ia treatment significantly decreased cell count by 63.8% when compared to the control. Verapamil drastically enhanced the growth inhibitory effect of 5-FU at all concentrations. At high-concentration 5-FU (50 m u g/ml), verapamil and hyperthermia had a synergistic growth-inhibitor y effect and caused a significant decrease in cell count by 81.4% in c omparison to the control. The modalities analyzed in this study requir e further investigation and have potential for clinical applicability to pancreatic cancer therapy in the future.