T. Ozaki et al., COMPLEX-FORMATION BETWEEN LAMIN-A AND THE RETINOBLASTOMA GENE-PRODUCT- IDENTIFICATION OF THE DOMAIN ON LAMIN-A REQUIRED FOR ITS INTERACTION, Oncogene, 9(9), 1994, pp. 2649-2653
The retinoblastoma susceptibility gene product (pRB) has been known to
function as a negative regulator of cell growth. Recent observations
suggest that its biological activity might be modulated by an interact
ion with nuclear structures. By using in vitro binding assays, we have
found that pRB can associate with lamin A, which has been known to be
one of the major nuclear matrix proteins. A series of GST-lamin A del
etion mutants was constructed to define the amino acid sequence requir
ed for binding to pRB. A GST-lamin A (247-355) contained an activity t
o associate with pRB, while the other constructs, such as GST-lamin A
(37-244) or GST-lamin A (356-571), could not bind to pRB. Within the p
RB-binding domain of lamin A, there exists the short amino acid sequen
ce which is also present in the pRB-binding region of the transcriptio
n factor E2F-1. The similar experiments using a set of GST-RB deletion
mutants revealed that a region containing the E1A-binding pocket B an
d the carboxy-terminal portion of pRB was responsible for binding to l
amin A.