THE EFFECTS OF GROWTH-INHIBITING TREATMENTS ON PHOTOREPAIR IN CULTURED FISH CELLS

Goldfish cells (RBCF-1) cultured at different cell densities were harv ested and their photorepair (PR) abilities were examined in terms of s urvival. Photorepair ability gradually increased during the phase of l ogarithmic growth, reaching a maximum in cells at the confluent state. This enhancement of PR ability disappeared 12 h after replating of ce lls in fresh medium. A number of growth-inhibiting treatments (serum d epletion, UVC, hydroxyurea [HU], change in incubation temperature) wer e tested for their ability to induce PR. The treatment of cells with H U and serum depletion induced PR while the other treatments did not. T he increase in the ability to perform PR after treatment with HU or se rum depletion returned to normal levels more rapidly than that after f luorescent light treatment.