The polysaccharide moiety of the O83 antigen (lipopolysaccharide, LPS)
consists of D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, an
d D-glucuronic acid in the molar ratios 1:2:1:1. Methylation analysis
of the polysaccharide and derived oligosaccharides as well as one- and
two-dimensional H-1 and C-13 NMR spectroscopy of the polysaccharide a
t pD 1 and 6 showed that the O83 polysaccharide has the primary struct
ure --> 6)-alpha-D-Glcp-(1 --> 4)-beta-D-GlcpA-(1 --> 6)-beta-D-Galp-(
1 --> 4)-beta-D-Galp-(1 --> 4)-beta-D-GlcpNAc-(1 -->