Lm. Chen et al., MOLECULAR-CLONING AND CHARACTERIZATION OF A NOVEL KALLIKREIN TRANSCRIPT IN COLON AND ITS DISTRIBUTION IN HUMAN TISSUES, Brazilian journal of medical and biological research, 27(8), 1994, pp. 1829-1838
1. We have cloned and characterized a new species of kallikrein cDNA f
rom a human colon cDNA library. The new kallikrein cDNA clone contains
a part of intron 2 of the tissue kallikrein gene which is spliced to
the remaining exon sequences. It does not contain exons 1 and 2. 2. An
in-frame open reading frame is present in the new kallikrein cDNA all
owing translation of a 216-amino acid product. The intron-containing k
allikrein transcript was detected in salivary glands, pancreas, kidney
, colon, prostate gland, testis, spleen, and lung by reverse-transcrip
tion/polymerase chain reaction followed by Southern blot analysis usin
g an intron-containing kallikrein-specific oligonucleotide probe. 3. T
he results indicate that the new species of kallikrein may be processe
d by alternative splicing or arises from a different transcription ini
tiation site.