Rl. Sammons et al., USE OF ENHANCED CHEMILUMINESCENCE TO QUANTIFY PROTEIN ADSORPTION TO CALCIUM-PHOSPHATE MATERIALS AND MICROCARRIER BEADS, Biomaterials, 15(10), 1994, pp. 842-847
The adsorption of serum proteins to calcium phosphate bone substitute
materials, positively-charged dextrose and negatively-charged polystyr
ene microcarrier beads was compared by SDS-PAGE (sodium dodecyl sulpha
te-polyacrylamide gel electrophoresis). Protein adsorption to hydroxya
patite (HA)-based materials was influenced by chemical composition. Su
rface charge sign, distribution and/or functional group affected prote
in adsorption to microcarrier beads. Enhanced chemiluminescence was us
ed to quantify adsorption of fibronectin and vitronectin following Wes
tern blotting, and to monitor the kinetics of adsorption of these two
proteins to HA and Biosilon. Relative to total protein, fluctuating le
vels of fibronectin were detected on both materials, in contrast, vitr
onectin adsorption increased over the course of the incubation period
with maximal relative adsorption detected after 30 min on Biosilon and
60 min on HA.