USE OF ENHANCED CHEMILUMINESCENCE TO QUANTIFY PROTEIN ADSORPTION TO CALCIUM-PHOSPHATE MATERIALS AND MICROCARRIER BEADS

The adsorption of serum proteins to calcium phosphate bone substitute materials, positively-charged dextrose and negatively-charged polystyr ene microcarrier beads was compared by SDS-PAGE (sodium dodecyl sulpha te-polyacrylamide gel electrophoresis). Protein adsorption to hydroxya patite (HA)-based materials was influenced by chemical composition. Su rface charge sign, distribution and/or functional group affected prote in adsorption to microcarrier beads. Enhanced chemiluminescence was us ed to quantify adsorption of fibronectin and vitronectin following Wes tern blotting, and to monitor the kinetics of adsorption of these two proteins to HA and Biosilon. Relative to total protein, fluctuating le vels of fibronectin were detected on both materials, in contrast, vitr onectin adsorption increased over the course of the incubation period with maximal relative adsorption detected after 30 min on Biosilon and 60 min on HA.