H. Mizoguchi et al., SPECIFIC BINDING OF 12-O-TETRADECANOYLPHORBOL-13-ACETATE TO HISTONE H1, Biological & pharmaceutical bulletin, 17(8), 1994, pp. 1114-1117
12-O-Tetradecanoylphorbol-13-acetate (TPA) binds specifically to histo
ne H1 prepared from HeLa cells and to calf histone H1 with dissociatio
n constants of 4.3 x 10(-7) and 9.3 x 10(-7) M1 respectively. The TPA-
binding to histone H1 was confirmed immunologically using a monoclonal
antibody directed against an evolutionally conserved epitope of histo
ne H1. This binding would influence DNA structure and the basal level
of transcription, and may, in turn, account for the cell growth-regula
tory activity of TPA.