SPECIFIC BINDING OF 12-O-TETRADECANOYLPHORBOL-13-ACETATE TO HISTONE H1

Authors
MIZOGUCHI H FUKASAWA H STEUERNAGEL A YANO K YAMASAKI M IWASAKI S HASHIMOTO Y
Citation
H. Mizoguchi et al., SPECIFIC BINDING OF 12-O-TETRADECANOYLPHORBOL-13-ACETATE TO HISTONE H1, Biological & pharmaceutical bulletin, 17(8), 1994, pp. 1114-1117
Citations number
26
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Biological & pharmaceutical bulletin → ACNP
ISSN journal
09186158
Volume
17
Issue
8
Year of publication
1994
Pages
1114 - 1117
Database
ISI
SICI code
0918-6158(1994)17:8<1114:SBO1TH>2.0.ZU;2-X
Abstract
12-O-Tetradecanoylphorbol-13-acetate (TPA) binds specifically to histo ne H1 prepared from HeLa cells and to calf histone H1 with dissociatio n constants of 4.3 x 10(-7) and 9.3 x 10(-7) M1 respectively. The TPA- binding to histone H1 was confirmed immunologically using a monoclonal antibody directed against an evolutionally conserved epitope of histo ne H1. This binding would influence DNA structure and the basal level of transcription, and may, in turn, account for the cell growth-regula tory activity of TPA.