IN-VIVO HYDROGEN-PEROXIDE PRODUCTION IN RAT REMNANT KIDNEY

In the rat remnant kidney hydrogen peroxide (H2O2) production is incre ased when compared to the normal kidney. The activities of the peroxis omal H2O2-producing oxidases, D-amino acid oxidase and acyl-coenzyme A oxidase, and of the extraperoxisomal superoxide dismutase are decreas ed in renal homogenate. The peroxisomal L-alpha-hydroxyacid oxidase an d L-lactate oxidase as well as the peroxisomal H2O2 scavenger catalase preserve their activity. The activity of the cytosolic scavenging sys tem, glutathione peroxidase, is decreased by 40%. A starvation period of 48 h does not produce a measurable increase in H2O2 production in t he normal nor in the remnant kidney. On visual inspection, peroxisomal morphology and distribution in the renal tubules are similar in the n ormal and remnant kidney tissue.