IMMOBILIZATION OF BROMOPEROXIDASE FROM CORALLINA-OFFICINALIS

The vanadium-dependent bromoperoxidase from the macroalga Corallina of ficinalis was immobilised on a cellulose acetate support with retentio n of approaching 50% of the applied units of activity. The enzyme exhi bited high thermal stability and retained activity in repeated use. Th e immobilised enzyme showed tolerance to organic solvents similar to t hat of the free enzyme in the case of methanol but differed for aceton e and ethanol, and with the latter showed enhanced activity as the % b y volume of the solvent was increased.