HPV TYPING OF CERVICAL SQUAMOUS LESIONS BY IN-SITU HPV DNA HYBRIDIZATION - INFLUENCE OF HPV TYPE AND THERAPY ON THE FOLLOW-UP OF LOW-GRADE SQUAMOUS CERVICAL DISEASE

Papanicolaou (Pap)-stained cervical specimens from 160 squamous lesion s were processed for the detection of human papillomavirus (HPV) DNA b y an in situ hybridization (ISH) assay. Three biotinylated HPV DNA pro bes were employed, each containing HPV genotypes 6/11, HPV genotypes 1 6/18, or HPV genotypes 31/35/51. The HPV etiology of 86 lesions was as certained (53.8%). In 74 out of 135 (58.8%) HPV-typed low-grade squamo us intraepithelial lesions (SILs), HPV 6/11 was found in nine (6.6%), HPV 16/18 in 46 (34.29%), and HPV 31/35/51 in 19 lesions (14.1%),- in 11 out of 18 HPV-typed high-grade SILs (61.1%), seven lesions (38.9%) were typed for HPV 16/18 and four (22.2%) for HPV 31/35/51. Of seven i nvasive carcinomas; only one (14.39%) reacted with the HPV 16/18 DNA p robe. A cohort of 124 low-grade SILs was followed cytologically for a year. The results of this study are discussed in light of HPV type ass ociation and therapy. (C) 1994 Wiley-Liss, Inc.