Lc. Wu et al., PLATELET-DERIVED GROWTH FACTOR-BB ACCELERATES WOUND CLOSURE IN A NEW MESENTERY CULTURE MODEL WITHOUT MACROPHAGES, Annals of plastic surgery, 33(2), 1994, pp. 155-161
Platelet-derived growth factor-BB has multiple effects in vitro and ha
s been demonstrated to accelerate wound healing in several animal mode
ls, but its precise mechanisms of action in vivo have not been fully e
xplained. A rat mesentery culture model was developed, and platelet-de
rived growth factor-BB (100 ng/ml) was added to the medium containing
2% fetal calf serum for examining its closure effects. The mesentery s
pecimens were stained by routine hematoxylin and eosin and immunohisto
chemical staining for macrophages. A significant improvement in closur
e rate and complete closure rate was seen when platelet-derived growth
factor-BB was added to the medium compared with the controls (p < 0.0
5). There are significantly fewer macrophages in the in vitro specimen
than in the in vivo specimen. In conclusion, platelet-derived growth
factor-BB can contribute to wound closure of a mesenteric defect even
without macrophages, suggesting a second mechanism in which it can act
directly on mesenchymal cells during wound healing. This tissue-cultu
re model is useful for gaining further insights into growth factor int
eractions and wound healing.