EXAMINING THE IMMUNOBIOLOGICAL PROPERTIES OF HYBRID PROTEINS CONSISTING OF ALPHA-TUMOR NECROTIC FACTOR AND ALPHA(1)-THYMOSINE

The preparations of alpha1-thymosine (T), alpha-tumor necrotic factor (TNF) and their based hybrid proteins: T-TNF, TNF-T, and T-TNF-T were studied by using a wide spectrum of immunobiological tests. In the L-9 29 cells, T-TNF preserved cytotoxicity unique to TNF; TNF-T preserved it 10 times less, and T-NTF-T was completely inactive. TNF-T inhibited the growth of Molt-4, Jm-9, Raji cells by 63=84%, and TNF suppressed only Raji cells by 50%. Hybrid proteins caused cell proliferation of t he thymus, spleen, and lymph nodes; H-2K. CD4, and CD8 differently inc reased the expression of thymocytic antigens. The authors found the di fferent effects of the drugs on phagocytosis, the production of antibo dy-forming cells, delayed reaction, and activation of natural killer c ells. The protein T-TNF-T produced the most pronounced action.