Hr. Lijnen et al., CHARACTERIZATION OF THE INTERACTION BETWEEN PLASMINOGEN AND STAPHYLOKINASE, European journal of biochemistry, 224(1), 1994, pp. 143-149
Binding parameters [association (k(a)) and dissociation (k(d)) rate co
nstants, and affinity constants (K-a = k(a)/k(d))] for the interaction
between recombinant staphylokinase (SakSTAR) and plasmin(ogen) were d
etermined by real-time biospecific interaction analysis. The K-a value
for binding of SakSTAR to native human Glu-plasminogen was 0.93X10(8)
M(-1) as compared to 2.0X10(8)M(-1) and 1.6X10(8)M(-1), respectively,
for the binding to [S741A]recombinant plasminogen or Lys-[S741A]recomb
inant plasminogen (intact or proteolytically degraded plasminogen with
the active site Ser741 replaced by alanine). Binding of SakSTAR to ac
tive plasmin or to active-site blocked plasmin occurred with K-a value
s of 4.0x10(8)M(-1) and 8.4X10(8)M(-1), respectively, whereas active-s
ite blocked LMM-plasmin (a plasmin derivative lacking kringles 1-4) an
d the plasmin B-chain bound with K-a values of 1.0x10(8)M(-1) and 0.49
X10(8)M(-1), respectively. Lysine-binding site I (a plasminogen deriva
tive consisting of kringles 1-3) and lysine-binding site II (a plasmin
ogen derivative consisting of kringle 4) bound with much lower affinit
y (K-a values of 1.2x10(5)M(-1) and 2.9x10(5)M(-1), respectively). The
binding of these plasminogen derivatives to streptokinase occurred wi
th similar relative K-a values. The K-a values for binding of the plas
min-SakSTAR complex to streptokinase and binding of the plasmin-strept
okinase complex to SakSTAR, were, respectively, 44-fold and 30-fold lo
wer than the values for free plasmin. The K-a for binding of plasminog
en to the inactive mutants [M26R]Sak42D or [M26A]Sak42D (site-specific
mutagenesis of Met26 to arginine or alanine) were 10-20-fold lower th
an that of native staphylokinase. These results indicate that: (a) the
affinity of staphylokinase for Glu-plasminogen and Lysplasminogen is
comparable; (b) the active site in the plasmin molecule is not require
d for binding; (c) kringle structures 1-4 of plasminogen do not contri
bute significantly to plasminogen binding of staphylokinase; (d) Met26
in staphylokinase is important for its high-affinity binding to plasm
inogen; (e) the binding sites on plasmin for staphylokinase and strept
okinase overlap at least partially.