MAPPING OF THE 12Q12-Q22 REGION WITH RESPECT TO TUMOR TRANSLOCATION BREAKPOINTS

The consistent involvement of the region 12q13-q15 in numerous human t umors speaks in favor of the presence of genes that may contribute to oncogenesis. Mapping genes within this region of chromosome 12 is a ne cessary step toward the identification of those that play a role in th is process. We have undertaken a multiplex analysis using translocatio n breakpoint mapping to order from the centromere to the telomere a se ries of 24 loci from the region 12q12-q22. Thirteen adipose tissue tum ors with seven different chromosome changes involving the long arm of chromosome 12 (12q) were used. Since most of these loci are genes or a nonymous DNA segments largely available to the scientific community, t his map should be useful for investigation of genetic disorders associ ated with chromosome 12q. While these breakpoints were used as natural landmarks to order groups of loci, this work has positioned them more accurately, leading to a better chromosomal definition of the translo cations than the one derived from standard cytogenetic studies. (C) 19 94 Academic Press, Inc.