ASSIGNMENT OF THE GENE ENCODING THE 5-HT1E SEROTONIN RECEPTOR (S31) (LOCUS HTR1E) TO HUMAN-CHROMOSOME 6Q14-Q15

The human gene for the 5-HT1E serotonin receptor was recently cloned, but no chromosomal assignment has yet been given to this gene (locus H TR1E). In this work, we demonstrate by two independent polymerase chai n reactions on a panel of human-hamster somatic cell hybrid genomic DN A that the 5-HT1E serotonin receptor gene is localized on human chromo some 6. Furthermore, by means of in situ hybridization to human metaph ase chromosomes, using the cloned 5-HT1E receptor gene (phage clone la mbda-S31; Levy et al., FEBS Lett. 296:201-206, 1992) as a probe, we de monstrate that this gene is localized to the q14-q15 region on chromos ome 6. Screening of genomic DNA from 15 unrelated Caucasian individual s, using as a probe the open reading frame of the cloned 5-HT1E recept or gene, did not reveal any restriction fragment length polymorphisms with the enzymes BamHI, BanII, BglII, EcoRI, HincII, HindIII, HinfI, M spI, PstI, and PvuII. Since the 5-HT1E receptor is found mainly in the cerebral cortex and abnormal function of the serotonergic system has been implicated in a variety of neurologic and psychiatric diseases, t he precise chromosomal assignment of the 5-HT1E receptor gene is the c rucial first step toward the evaluation of this locus as a candidate f or mutations in such syndromes. (C) 1994 Academic Press, Inc.