Dg. Allison et Rd. Nolan, INFLUENCE OF GROWTH-RATE AND NUTRIENT LIMITATION ON MONOBACTAM PRODUCTION AND PEPTIDOGLYCAN SYNTHESIS IN PSEUDOMONAS-AERUGINOSA, Journal of basic microbiology, 34(4), 1994, pp. 217-224
The effects of growth rate and nutrient limitation on monobactam produ
ction, peptidoglycan content and mean cell length in Pseudomonas aerug
inosa was studied using continuous culture techniques. All three param
eters increased progressively with growth rate, a greater response bei
ng shown under carbon limitation compared to that occurring under nitr
ogen limiting conditions. interestingly, monobactum production mirrore
d peptidoglycan synthesis. In addition, the monobactam exhibited a bro
ad rang of antibacterial activity and bound preferentially to PBP 1A i
n the producing organism. Moreover, addition of the monobactam to a gr
owing culture inhibited cell wall synthesis. These results are discuss
ed in relation to the control and regulation of peptidoglycan synthesi
s.