A REVISED MAP LOCATION FOR THE HISTIDINE UTILIZATION GENES IN PSEUDOMONAS-PUTIDA

The histidine utilization genes hutH and hutU of Pseudomonas putida AT CC 12633 have been mapped by interrupted mating and transduction to a location at approximately 43 minutes on the chromosome, closely linked to ser-800 and met-400 markers previously shown to be at 46 and 42 mi nutes, respectively. Since restriction enzyme mapping and cloning resu lts have established thar all genes associated with the hut pathway ar e contiguous, earlier maps of this strain which place these genes near 10 minutes on the chromosome in a superoperonic catabolic cluster are in error.