A. Purohit et al., THE HYDROLYSIS OF ESTRONE SULFATE AND DEHYDROEPIANDROSTERONE-SULFATE BY HUMAN STEROID SULFATASE EXPRESSED IN TRANSFECTED COS-1 CELLS, Journal of steroid biochemistry and molecular biology, 50(1-2), 1994, pp. 101-104
Whether the same or distinct steroid sulphatases (STS) are involved in
the hydrolysis of alkyl and aryl steroid sulphates remains controvers
ial. We have examined the ability of a placental steroid sulphatase to
hydrolyse oestrone sulphate and/or dehydroepiandrosterone sulphate (D
HA-S) by expressing the enzyme in COS-1 cells. Using either intact cel
ls or broken cell preparations, the expressed sulphatase was found to
hydrolyse both oestrone sulphate and DHA-S. The catalysis of oestrone
sulphate and DHA-S by the expressed sulphatase was almost completely a
bolished by the steroid sulphatase inhibitor, oestrone-3-O-sulphamate.
It is concluded that both alkyl and aryl steroid sulphates can be hyd
rolysed by the same steroid sulphatase.