S. Berrada et al., EFFECT OF A DERIVATIZED DEXTRAN ON HUMAN OSTEOBLAST GROWTH AND PHENOTYPE EXPRESSION, Journal of biomaterials science. Polymer ed., 6(2), 1994, pp. 211-222
Water soluble derivatized dextran named E9 with a molecular weight of
45 000 g l-1 containing 58% methyl carboxylic acid unit, 19% benzylami
de unit, and 26% sulfonate with a specific anticoagulant activity of 0
.29 IU m g-1 was studied for its effects on human osteoblast growth an
d phenotype expression for short-term treatment. At concentrations bet
ween 1 ng ml-1 and 1 mug ml-1 E9 has no effect on DNA synthesis wherea
s at higher concentrations DNA synthesis is inhibited in a dose relate
d fashion (87% for 400 mg ml-1). For concentrations which do not modif
y osteoblast growth, E9 promotes alkaline phosphatase activity, type I
collagen and osteocalcin synthesis with a maximum effect for 0.1-1 mu
g ml-1. It has a synergistic effect with hPTH increasing AMPc. Moreove
r, osteonectin synthesis was enhanced in a dose-dependent manner betwe
en 0.1 and 5 mug ml-1. These results seem to indicate that E9 is able
to stimulate human osteoblast phenotype expression and could be useful
in clinical applications.