A GENE ENCODING ENDO-1,4-BETA-GLUCANASE FROM BACILLUS SP 22-28

Clones of a gene encoding an endo-1,4-beta-glucanase (EC 3.2.1.4) were obtained from Bacillus sp. 22-28, and the nucleotides were sequenced , A recombinant plasmid, pMK5, included a complete ORF of 2352 bp that encoded 783 amino acid residues, Another plasmid, pM3, which showed e nzymatic activity in E. coli JM109, was also obtained, and it included an incomplete ORF of 1873 bp, which lacked the original stop codon an d 479 bp of the C-terminal region, The enzymes purified from both of t he two types of transformants have shown almost the same properties in comparison with that of the wild type Bacillus sp. 22-28.